Detection of mutations in the Plasmodium falciparum dihydrofolate reductase (dhfr) gene by dot-blot hybridization.

@article{AbdelMuhsin2002DetectionOM,
  title={Detection of mutations in the Plasmodium falciparum dihydrofolate reductase (dhfr) gene by dot-blot hybridization.},
  author={Abdel-Muhsin A. Abdel-Muhsin and Lisa C Ranford-Cartwright and Abdel-Rahman Medani and Salah Ahmed and Suad Suleiman and B. B. Khan and Paul N. Hunt and David Walliker and Hamza A. Babiker},
  journal={The American journal of tropical medicine and hygiene},
  year={2002},
  volume={67 1},
  pages={24-7}
}
There is a need for a specific, sensitive, robust, and large-scale method for diagnosis of drug resistance genes in natural Plasmodium falciparum infections. Established polymerase chain reaction (PCR)-based methods may be compromised by the multiplicity of P. falciparum genotypes in natural infections. Here we adopt a dot-blot method to detect point mutations at nucleotide 323 (residue 108) in the P. falciparum dihydrofolate reductase (dhfr) gene using allele-specific oligonucleotide probes… CONTINUE READING

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High recombination rate in natural populations of Plasmodium falciparum.

Proceedings of the National Academy of Sciences of the United States of America • 1999

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