Detection of multidrug-resistant Salmonella typhimurium DT104 by multiplex polymerase chain reaction.

@article{Khan2000DetectionOM,
  title={Detection of multidrug-resistant Salmonella typhimurium DT104 by multiplex polymerase chain reaction.},
  author={Ashraf A. Khan and Mohamed S. Nawaz and Saeed Ahmad Khan and Carl Cerniglia},
  journal={FEMS microbiology letters},
  year={2000},
  volume={182 2},
  pages={
          355-60
        }
}
Salmonella typhimurium definitive type 104 (DT104) is a virulent pathogen for humans and animals with many strains having multiple drug resistance characteristics. The organism typically carries resistance to ampicillin, chloramphenicol, florfenicol, streptomycin, sulfonamides, and tetracycline (ACSSuT-resistant). A multiplex PCR method was developed to simultaneously amplify four genes, florfenicol (flo(st)), virulence (spvC), invasion (invA), and integron (int) from S. typhimurium DT104… CONTINUE READING

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