Detection of minimal residual disease in leukaemia by RT-PCR.

Abstract

Reverse transcription-PCR (RT-PCR) describes a technique whereby RNA is first reverse transcribed into complementary DNA (cDNA) using the enzyme reverse transcriptase, and the resulting cDNA amplified in either a single-step or a two-step nested PCR reaction. It is particularly useful for detecting molecular markers associated with leukaemia, as it enables… (More)
DOI: 10.1007/978-1-60761-947-5_18

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