Detection of mineralized structures in early stages of development of marine Teleostei using a modified alcian blue-alizarin red double staining technique for bone and cartilage.

@article{Gavaia2000DetectionOM,
  title={Detection of mineralized structures in early stages of development of marine Teleostei using a modified alcian blue-alizarin red double staining technique for bone and cartilage.},
  author={Paulo J. Gavaia and Carmen Sarasquete and Maria Leonor Cancela},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  year={2000},
  volume={75 2},
  pages={79-84}
}
We have developed a procedure for staining cartilage and bone in fish larvae as small as 2 mm (notochord length), for which standard alcian blue/alizarin red procedures did not give positive and/or consistent results. Small calcified structures only 100-200 microns in length can be clearly visualized. The method is suitable for both ontogenic studies during early stages of skeletal development in most marine fishes (e.g., Sparus aurata L., Solea senegalensis Kaup), whose larvae at hatching are… CONTINUE READING

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