Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes

@article{Jung1999DetectionOM,
  title={Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes},
  author={Roman Jung and K Petersen and William H Kr{\"u}ger and Marion Wolf and Christoph Wagener and Axel Rolf Zander and Michael Neumaier},
  journal={British Journal of Cancer},
  year={1999},
  volume={81},
  pages={870 - 873}
}
The reverse transcription polymerase chain reaction (RT-PCR) amplification of cytokeratin 20 (CK20) mRNA is considered a promising candidate method for the detection of circulating tumour cells in bone marrow and peripheral blood of cancer patients. In this study we have investigated the diagnostic specificity of the CK20 mRNA detection in samples from healthy donors (HD; n = 33), intensive care units patients (ICU; n = 20) and bone marrow obtained from patients suffering from chronic… CONTINUE READING