Detection of microRNAs in plants by in situ hybridisation.

  title={Detection of microRNAs in plants by in situ hybridisation.},
  author={{\'E}va V{\'a}rallyay and Zolt{\'a}n Havelda},
  journal={Methods in molecular biology},
MicroRNAs (miRNAs) are short, about 21 nucleotides in length, non-coding, regulatory RNA molecules representing a new layer in post-transcriptional gene expression regulation. Spatial and temporal analysis of miRNA accumulation by in situ analyses is the prerequisite of understanding the precise biological functions of miRNAs. Since miRNAs are very short molecules, their in situ analysis is technically demanding. Our method is based on the usage of highly sensitive LNA-modified oligonucleotide… CONTINUE READING