Detection of methionine-enkephalin at the 10(-16) mole level.

Abstract

A high performance radioimmunoassay for Met5-enkephalin has been developed, using two ways to circumvent the difficulties of obtaining such an assay for the opiate peptides. First, hemocyanin was used as the carrier for the antigen and secondly, a new iodination method for Met5-enkephalin was established, resulting in a [125I]probe with an intact methionyl… (More)

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