Detection of methane and quantification of methanogenic archaea in faeces from young broiler chickens using real-time PCR.

@article{Saengkerdsub2007DetectionOM,
  title={Detection of methane and quantification of methanogenic archaea in faeces from young broiler chickens using real-time PCR.},
  author={Suwat Saengkerdsub and Portero Herrera and Catherine L. Woodward and R C Anderson and David J. Nisbet and Steven C. Ricke},
  journal={Letters in applied microbiology},
  year={2007},
  volume={45 6},
  pages={
          629-34
        }
}
AIMS To detect the presence of methanogens in the faeces of broiler chicks during the first 2 weeks of age. METHODS AND RESULTS Chicken faecal samples from 120 broiler chicks were incubated for methane gas formation and methanogenic archaea were analysed using real-time PCR. The copy number of the order Methanobacteriales 16S rDNA gene in chicken faeces when the broilers were 3-12 days of age, litter and house flies collected in the bird house ranged from 4.19 to 5.51 log(10) g(-1) wet weight… CONTINUE READING

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