Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene.

@article{Doyle2005DetectionOM,
  title={Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene.},
  author={Carrie K Doyle and Marcelo B Labruna and Edward B Breitschwerdt and Yi-Wei Tang and Richard E Corstvet and Barbara C. Hegarty and Karen C. Bloch and Ping Li and David H Walker and Jere W McBride},
  journal={The Journal of molecular diagnostics : JMD},
  year={2005},
  volume={7 4},
  pages={504-10}
}
Ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses, in addition to causing serious and fatal infections in companion animals and livestock. We developed the first tricolor TaqMan real-time polymerase chain reaction assay capable of simultaneously detecting and discriminating medically important ehrlichiae in a single reaction. Analytical sensitivity of 50 copies per reaction was attained with templates from Ehrlichia chaffeensis, Ehrlichia… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 43 extracted citations

Similar Papers

Loading similar papers…