Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay.

@article{Aldeguer2014DetectionOM,
  title={Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay.},
  author={Miriam Aldeguer and Mar{\'i}a L{\'o}pez-Andreo and Jos{\'e} Antonio Gabald{\'o}n and A. Puyet},
  journal={Food chemistry},
  year={2014},
  volume={145},
  pages={1086-91}
}
A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin × tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the… CONTINUE READING

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