Detection of low levels of Listeria monocytogenes cells by using a fiber-optic immunosensor.

@article{Geng2004DetectionOL,
  title={Detection of low levels of Listeria monocytogenes cells by using a fiber-optic immunosensor.},
  author={Tao Geng and Mark T. Morgan and Arun K. Bhunia},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 10},
  pages={6138-46}
}
Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture Listeria cells on the fiber. Capture of… CONTINUE READING
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