Detection of living cells in stratospheric samples

@inproceedings{Harris2002DetectionOL,
  title={Detection of living cells in stratospheric samples},
  author={Melanie J. Harris and N. Chandra Wickramasinghe and David Lloyd and Jayant V. Narlikar and Premraj Rajaratnam and Michael P. Turner and Shirwan Al-Mufti and Max K. Wallis and Sivasankari Ramadurai and Fred Sir Hoyle},
  booktitle={SPIE Optics + Photonics},
  year={2002}
}
Air samples collected aseptically over tropical India at various stratospheric altitudes ranging from 20 to 41 km using cryosampler assemblies carried on balloons flown from Hyderabad have shown evidence of living microbial cells. Unambiguous evidence of living cells came from examining micropore filters on which the samples were recovered with the use of voltage sensitive lipophilic dyes that could detect the presents of active cells. Clumps of viable cells were found at all altitudes using… 
Microorganisms cultured from stratospheric air samples obtained at 41 km.
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ISOLATION OF A DIATOM FRUSTULE FRAGMENT FROM THE LOWER STRATOSPHERE (22-27Km)-EVIDENCE FOR A COSMIC ORIGIN
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  • G. Konesky
  • Physics
    Optical Engineering + Applications
  • 2010
Microorganisms may exist in Low Earth Orbit both from terrestrial sources, and potentially, from extraterrestrial origins. A simple, low cost method for in-situ detection is proposed, suitable for
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DNA Damage and Survival Time Course of Deinococcal Cell Pellets During 3 Years of Exposure to Outer Space
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The results indicated the importance of the aggregated form of cells for surviving in harsh space environment and support the possibility of microbial cell aggregates (pellets) as an ark for interplanetary transfer of microbes within several years.
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