Detection of living cells in stratospheric samples

  title={Detection of living cells in stratospheric samples},
  author={Melanie J. Harris and N. Chandra Wickramasinghe and David Lloyd and Jayant V. Narlikar and Premraj Rajaratnam and Michael P. Turner and Shirwan Al-Mufti and Max K. Wallis and Sivasankari Ramadurai and Fred Sir Hoyle},
  booktitle={SPIE Optics + Photonics},
Air samples collected aseptically over tropical India at various stratospheric altitudes ranging from 20 to 41 km using cryosampler assemblies carried on balloons flown from Hyderabad have shown evidence of living microbial cells. Unambiguous evidence of living cells came from examining micropore filters on which the samples were recovered with the use of voltage sensitive lipophilic dyes that could detect the presents of active cells. Clumps of viable cells were found at all altitudes using… 
Microorganisms cultured from stratospheric air samples obtained at 41 km.
It is confident that the organisms originated from the stratosphere, and possible mechanisms by which these organisms could have attained such a height are discussed.
Study of putative microfossils in space dust from the stratosphere
Abstract Interplanetary dust particles (IDPs) were recovered from the stratosphere by a cryosampler flown below a balloon flying at altitudes of 20–41 km. The present study uses high-resolution
Recovery of Cometary Microorganisms from the Stratosphere
The Hoyle-Wickramasinghe theory of cometary panspermia posits that terrestrial life was introduced by comets, and predicts that this process can be tested by the detection of an ongoing incidence
Assessing Panspermia Hypothesis by Microorganisms Collected from The High Altitude Atmosphere
From limited knowledge available to date, it is suggested that terrestrial microorganisms may have had chances to be ejected and transferred to outer space and survival of these organisms during their space travel and proliferation on other planets might be also feasible.
Stratospheric microbiology at 20 km over the Pacific Ocean
It is unlikely that the samples collected indicate a permanent, stratospheric ecosystem, however, the presence of viable fungi and bacteria in transoceanic stratosphere remains relevant to understanding the distribution and extent of microbial life on Earth.
Sampling of the stratosphere at heights between 22 and 27 km was carried out in the UK on 31st July 2013 using balloon-borne equipment carrying aseptically clean electron microscope stubs onto which
Biological entities isolated from the stratosphere (22-27km): case for their space origin
Biological entities were isolated at a height of between 22-27 km in the stratosphere. Sampling of this region was carried out in the UK in July 2013 using a relatively simple low-cost balloon-borne
Proposed mission for in-situ detection of microorganisms in Low Earth Orbit
  • G. Konesky
  • Physics
    Optical Engineering + Applications
  • 2010
Microorganisms may exist in Low Earth Orbit both from terrestrial sources, and potentially, from extraterrestrial origins. A simple, low cost method for in-situ detection is proposed, suitable for
Masses Staining Positive for DNA-Isolated from the Stratosphere at a Height of 41 km
It is suggested that the masses are arriving to the stratosphere from space and not upcoming from Earth; evidence is provided to support this view.
DNA Damage and Survival Time Course of Deinococcal Cell Pellets During 3 Years of Exposure to Outer Space
The results indicated the importance of the aggregated form of cells for surviving in harsh space environment and support the possibility of microbial cell aggregates (pellets) as an ark for interplanetary transfer of microbes within several years.


Vigour, vitality and viability of microorganisms
For suspended organisms, flow cytometry provides a powerful means of measurement of a wide range of characteristics and for microbes in aggregates or growing on surfaces may be obtained by use of confocal scanning laser microscopy.
Astronomical Origins of Life, (Kluwer
  • 2000
Use of two oxonols and a fluorescent tetrazolium dye to monitor starvation of E.coli in sea water by flow symmetry
  • J. Microbiology Met/i
  • 1995
Use of two oxonols and a fluorescent tetrazolium dye to monitor starvation ofE.coli in sea water by flow symmetry
  • J. Microbiology Meth
  • 1995
Meteors" in Cosmic Dust (ed
  • J.A.M. McDonnell) p
  • 1978
Meteors" in Cosmic L)ut (ed
  • J.A.M. McDonnell)
  • 1978
Airborne Microbes. Symposium of the Society for Microbiology
  • (eds P.H. Gregory and J.L Monteith)
  • 1967