Detection of lipofuscin-like fluorophore in oxidized human low-density lipoprotein. 4-hydroxy-2-nonenal as a potential source of fluorescent chromophore.

@article{Itakura2000DetectionOL,
  title={Detection of lipofuscin-like fluorophore in oxidized human low-density lipoprotein. 4-hydroxy-2-nonenal as a potential source of fluorescent chromophore.},
  author={Koichi Itakura and Tomoko Oya-Ito and Toshihiko Osawa and Seiji Yamada and Shinya Toyokuni and Noriyuki Shibata and Makio Kobayashi and Koji Uchida},
  journal={FEBS letters},
  year={2000},
  volume={473 2},
  pages={249-53}
}
It has recently been shown that the lipid peroxidation product 4-hydroxy-2-nonenal (HNE) forms a fluorescent hydroxyiminodihydropyrrole derivative with the epsilon-amino group of lysine residue. In this study, we raised a monoclonal antibody (mAb2C12) directed to the fluorophore-protein conjugate and found that the antibody was specific to the chromophore structure of the compound. Immunohistochemical analysis of atherosclerotic lesions from the human aorta showed that the fluorophore was… CONTINUE READING
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