Detection of leptospiral DNA by nucleic acid hybridisation with 32P- and biotin-labelled probes.

@article{Terpstra1986DetectionOL,
  title={Detection of leptospiral DNA by nucleic acid hybridisation with 32P- and biotin-labelled probes.},
  author={Wiepko J. Terpstra and Gerard J. Schoone and Jan ter Schegget},
  journal={Journal of medical microbiology},
  year={1986},
  volume={22 1},
  pages={
          23-8
        }
}
Dot hybridisation with 32P- and biotin-labelled probes prepared from leptospiral DNA was performed to develop a sensitive and specific diagnostic method for early infection with leptospires. The smallest amounts of leptospiral DNA that could be detected with 32P- and biotin-labelled probes was 1.5 pg and 5 pg, respectively, corresponding to about 750 and 2500 leptospires. Dot hybridisation with a 32P-labelled probe detected leptospiral DNA in sera from all of 14 experimentally infected golden… 
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57 Citations
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Methods Citations
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57 Citations

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The results indicate that specific recombinant DNA probes might provide tools for routine diagnosis and classification in cases of hardjo infections and show a high sensitivity of the assays but also considerable cross-hybridization.
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Detection of seven species of pathogenic leptospires by PCR using two sets of primers.
Two sets of primers derived from genomic DNA libraries of Leptospira serovars icterohaemorrhagiae (strain RGA) and bim (strain 1051) enabled the amplification by PCR of target DNA fragments from…
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