Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation

@article{Wang2004DetectionOI,
  title={Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation},
  author={Z. Wang and P. Troilo and X. Wang and T. G. Griffiths and S. Pacchione and A. B. Barnum and L. B. Harper and C. Pauley and Z. Niu and L. Denisova and T. Follmer and G. Rizzuto and G. Ciliberto and E. Fattori and N. L. Monica and S. Manam and B. Ledwith},
  journal={Gene Therapy},
  year={2004},
  volume={11},
  pages={711-721}
}
Plasmid vectors have been widely used for DNA vaccines and gene therapy. Following intramuscular injection, the plasmid that persists is extrachromosomal and integration into host DNA, if it occurs at all, is negligible. However, new technologies for improving DNA delivery could increase the frequency of integration. In the present study, we tested the effect of electroporation on plasmid uptake and potential integration following intramuscular injection in mice, using a plasmid containing the… Expand

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References

SHOWING 1-10 OF 28 REFERENCES
Plasmid DNA vaccines: assay for integration into host genomic DNA.
Potential DNA Vaccine Integration into Host Cell Genome
In vivo DNA electrotransfer.
Electro-Gene-Transfer: A New Approach for Muscle Gene Delivery
Illegitimate DNA integration in mammalian cells
...
1
2
3
...