Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation

@article{Wang2004DetectionOI,
  title={Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation},
  author={Zhi-Peng Wang and Philip J Troilo and Xiaolong Wang and Thomas L. Griffiths and Stephen J Pacchione and A. C. B. Barnum and Licia Harper and Cindy J Pauley and Z Niu and Lyudmila Denisova and Thy Follmer and Gabriella Rizzuto and Gennaro Ciliberto and Elena Fattori and Nicola La Monica and S. R. Manam and Brian J Ledwith},
  journal={Gene Therapy},
  year={2004},
  volume={11},
  pages={711-721}
}
Plasmid vectors have been widely used for DNA vaccines and gene therapy. Following intramuscular injection, the plasmid that persists is extrachromosomal and integration into host DNA, if it occurs at all, is negligible. However, new technologies for improving DNA delivery could increase the frequency of integration. In the present study, we tested the… CONTINUE READING