Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation

  title={Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation},
  author={Z Wang and Philip J. Troilo and X. Wang and Thomas G. Griffiths and Stephen J. Pacchione and Amy B. Barnum and Laural B. Harper and Cindy J. Pauley and Zhutian Niu and Lyudmila Denisova and Thy T. Follmer and Gabriella Rizzuto and Gennaro Ciliberto and Elena Fattori and N La Monica and Sujata Manam and Brian J. Ledwith},
  journal={Gene Therapy},
Plasmid vectors have been widely used for DNA vaccines and gene therapy. Following intramuscular injection, the plasmid that persists is extrachromosomal and integration into host DNA, if it occurs at all, is negligible. However, new technologies for improving DNA delivery could increase the frequency of integration. In the present study, we tested the effect of electroporation on plasmid uptake and potential integration following intramuscular injection in mice, using a plasmid containing the… 

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