Detection of in vivo-induced IL-1 mRNA in murine cells by flow cytometry (FC) and fluorescent in situ hybridization (FISH).

@article{Pennline1992DetectionOI,
  title={Detection of in vivo-induced IL-1 mRNA in murine cells by flow cytometry (FC) and fluorescent in situ hybridization (FISH).},
  author={K. J. Pennline and F Pellerito-Bessette and Shelby P. Umland and Marvin Siegel and Sidney R. Smith},
  journal={Lymphokine and cytokine research},
  year={1992},
  volume={11 1},
  pages={65-71}
}
Flow cytometry (FC) and fluorescent in situ hybridization (FISH) were used to detect in vivo induced IL-1 alpha (a) messenger RNA. Spleen cells and thioglycollate-induced peritoneal exudate cells (PEC) were harvested from Balb/C mice three hours after intraperitoneal injection of 20 micrograms LPS. Cells from each population were phenotyped for MAC or IAd… CONTINUE READING