Detection of in vitro kinase generated protein phosphorylation sites using gamma[18O4]-ATP and mass spectrometry.

@article{Zhou2007DetectionOI,
  title={Detection of in vitro kinase generated protein phosphorylation sites using gamma[18O4]-ATP and mass spectrometry.},
  author={Ming Zhou and Zhaojing Meng and Andrew G. Jobson and Yves Pommier and Timothy D. Veenstra},
  journal={Analytical chemistry},
  year={2007},
  volume={79 20},
  pages={7603-10}
}
A novel stable-isotope labeling approach for identification of phosphopeptides that utilizes adenosine triphosphate, in which four oxygen-16 atoms attached to the terminal phosphate group are substituted with oxygen-18 [gamma((18)O4)-ATP], has been developed. The ability to use gamma((18)O4)-ATP to monitor phosphorylation modification within various proteins was conducted by performing in vitro kinase reactions in the presence of a 1:1 mixture of gamma((18)O4)-ATP and normal isotopic abundance… CONTINUE READING