Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry.

@article{Schlosser2003DetectionOI,
  title={Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry.},
  author={Gitta Schlosser and Gabriella Pocsfalvi and Antonio Malorni and Angel Puerta and Mercedes de Frutos and K{\'a}roly V{\'e}key},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2003},
  volume={17 24},
  pages={2741-7}
}
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect an immune complex formed between beta-lactoglobulin and polyclonal anti-beta-lactoglobulin antibody in the gas phase. The most important experimental parameters to detect such a specific antibody-antigen complex by MALDI were the use of solutions at near-neutral pH and of sinapinic acid matrix prepared by the dried-droplet method. Under such conditions, predominantly one but also two molecules of antigen… CONTINUE READING