Detection of immobilized amplicons by ELISA-like techniques.

@article{Oroskar1996DetectionOI,
  title={Detection of immobilized amplicons by ELISA-like techniques.},
  author={A A Oroskar and Svend Erik Rasmussen and Heike Rasmussen and Stella R Rasmussen and Brenda M. Sullivan and A E Johansson},
  journal={Clinical chemistry},
  year={1996},
  volume={42 9},
  pages={1547-55}
}
The NucleoLink surface is a physically modified, thermostable, optically clear resin. It allows the covalent binding of 5'-phosphorylated oligonucleotides. Target DNA amplification by polymerase chain reaction (PCR) is accomplished by asymmetric amplification on the covalently immobilized primer that develops into immobilized amplicons. A DNA fragment of bovine leukemia virus is used as a model system for the detection of immobilized amplicons by ELISA-like techniques. Covalently bound… CONTINUE READING
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