Detection of immobilized amplicons by ELISA-like techniques.

  title={Detection of immobilized amplicons by ELISA-like techniques.},
  author={A. A. Oroskar and S. Rasmussen and H. Rasmussen and S. R. Rasmussen and B. Sullivan and A. Johansson},
  journal={Clinical chemistry},
  volume={42 9},
  • A. A. Oroskar, S. Rasmussen, +3 authors A. Johansson
  • Published 1996
  • Chemistry, Medicine
  • Clinical chemistry
  • The NucleoLink surface is a physically modified, thermostable, optically clear resin. It allows the covalent binding of 5'-phosphorylated oligonucleotides. Target DNA amplification by polymerase chain reaction (PCR) is accomplished by asymmetric amplification on the covalently immobilized primer that develops into immobilized amplicons. A DNA fragment of bovine leukemia virus is used as a model system for the detection of immobilized amplicons by ELISA-like techniques. Covalently bound… CONTINUE READING
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