Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation.

@article{Masaki1995DetectionOH,
  title={Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation.},
  author={Hitoshi Masaki and Toshiko Atsumi and Hiroaki Sakurai},
  journal={Biochemical and biophysical research communications},
  year={1995},
  volume={206 2},
  pages={474-9}
}
The purpose of this study was to identify the active oxygen species generated in murine skin fibroblasts under UVB irradiation. When fibroblasts were exposed to UV light (UVA + UVB), hydroxyl radicals were detectable by ESR-spin trapping using DMPO as a spin trapping agent. Cell damage induced by UVB irradiation was prevented by addition of DMSO, a typical hydroxyl radical scavenger. Fibroblasts treated with a catalase inhibitor 3-amino-1H-1,2,4-triazole were more sensitive to UVB irradiation… CONTINUE READING

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