Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon.


An immunoprecipitation technique, which uses radiolabeled human gamma interferon (HuIFN-gamma) and a sheep anti-mouse F(ab')2 serum, is described for the rapid detection of mouse monoclonal antibodies to IFN-gamma in hybridoma culture supernatants. This technique which can be performed in 4 h detects both neutralizing and nonneutralizing antibodies to IFN… (More)


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