Detection of human respiratory syncytial virus in respiratory samples by LightCycler reverse transcriptase PCR.

@article{Whiley2002DetectionOH,
  title={Detection of human respiratory syncytial virus in respiratory samples by LightCycler reverse transcriptase PCR.},
  author={David Mark Whiley and Melanie W. Syrmis and Ian M Mackay and Theo P. Sloots},
  journal={Journal of clinical microbiology},
  year={2002},
  volume={40 12},
  pages={4418-22}
}
Laboratory diagnosis of human respiratory syncytial virus (hRSV) infections has traditionally been performed by virus isolation in cell culture and the direct fluorescent-antibody assay (DFA). Reverse transcriptase PCR (RT-PCR) is now recognized as a sensitive and specific alternative for detection of hRSV in respiratory samples. Using the LightCycler instrument, we developed a rapid RT-PCR assay for the detection of hRSV (the LC-RT-PCR) with a pair of hybridization probes that target the hRSV… CONTINUE READING
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Respiratory syncytial virus and parainfluenza virus

  • C. B. Hall
  • N. Engl. J. Med
  • 2001
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