Detection of human immunodeficiency virus (HIV) by colorimetric assay for reverse transcriptase activity on magnetic beads.

Abstract

A colorimetric assay for detection of reverse transcriptase (RT) of the human immunodeficiency virus (HIV) was developed using oligodeoxythymidylic acid (oligo-dT)-linked magnetic beads and digoxigenin-deoxyuridine triphosphate (dig-dUTP). During the RT reaction, dig-dUTP was incorporated into oligo-dT which had been hybridized to polyadenylic acid [poly (A… (More)

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@article{Suzuki1993DetectionOH, title={Detection of human immunodeficiency virus (HIV) by colorimetric assay for reverse transcriptase activity on magnetic beads.}, author={Kazuo Suzuki and Barbara P. Craddock and Naoyuki Okamoto and Tsuneo Kano and Roy T. Steigbigel}, journal={Biotechnology and applied biochemistry}, year={1993}, volume={18 ( Pt 1)}, pages={37-44} }