Detection of human cytomegalovirus in urine by DNA-DNA and RNA-DNA hybridization.

@article{Schuster1986DetectionOH,
  title={Detection of human cytomegalovirus in urine by DNA-DNA and RNA-DNA hybridization.},
  author={Volker Schuster and Bertfried Matz and Herbert Wiegand and Berthold Traub and Dione Kampa and Dieter Neumann-Haefelin},
  journal={The Journal of infectious diseases},
  year={1986},
  volume={154 2},
  pages={309-14}
}
A diagnostic hybridization assay for detecting human cytomegalovirus (HCMV) DNA in urine specimens was developed by using cloned viral DNA and in vitro-synthesized RNA probes. Both probes detected 3 pg of homologous DNA and hybridized with DNA of HCMV but not with other viral or human cellular DNA tested. In 95 urine specimens simultaneously tested by cell culture, the sensitivity of hybridization was at least 83%, and the specificity was at least 92%. This assay will be useful for rapid viral… CONTINUE READING