Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay: development and validation.

@article{Green1991DetectionOH,
  title={Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay: development and validation.},
  author={Elaine K Green and Stephen C. Bain and Philip J. Day and Anthony Barnett and F Charleson and Alan F. Jones and M R Walker},
  journal={Clinical chemistry},
  year={1991},
  volume={37 7},
  pages={1263-8}
}
A polymerase chain reaction (PCR) assay has been developed and validated by using allele-specific oligonucleotide (ASO) primers to specifically amplify E3, E2, and E4 polymorphic sequences of the human apolipoprotein E (apo E) genes. Degenerate ASOs containing one or two additional 3' mismatches provided greater specificity than did ASOs containing a single… CONTINUE READING