Detection of hormone release from individual cells in mixed populations using a reverse hemolytic plaque assay.

@article{Neill1983DetectionOH,
  title={Detection of hormone release from individual cells in mixed populations using a reverse hemolytic plaque assay.},
  author={Jimmy D. Neill and L. Stephen Frawley},
  journal={Endocrinology},
  year={1983},
  volume={112 3},
  pages={1135-7}
}
Prolactin (Prl) secreting cells in a mixed pituitary cell culture form microscopically-identifiable plaques (zones of hemolysis around the lactotropes) when incubated in a monolayer with staphylococcal protein-A-coated ovine erythrocytes in the presence of Prl antiserum and complement. Plaques form first at 15-30 min and are maximal in size and number at 2 h. Approximately 70% of the adenohypophyseal cells form plaques under these conditions. TRH increases, and dopamine decreases, the size and… CONTINUE READING
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