Detection of histidine oxidation in a monoclonal immunoglobulin gamma (IgG) 1 antibody.

@article{Amano2014DetectionOH,
  title={Detection of histidine oxidation in a monoclonal immunoglobulin gamma (IgG) 1 antibody.},
  author={Masato Amano and Naoki Kobayashi and Masayuki Yabuta and Susumu Uchiyama and Kiichi Fukui},
  journal={Analytical chemistry},
  year={2014},
  volume={86 15},
  pages={7536-43}
}
Although oxidation of methionine and tryptophan are known as popular chemical modifications that occur in monoclonal antibody (mAb) molecules, oxidation of other amino acids in mAbs has not been reported to date. In this study, oxidation of the histidine residue in a human immunoglobulin gamma (IgG) 1 molecule was discovered for the first time by mass spectrometry. The oxidation of a specific histidine located at the CH2 domain of IgG1 occurred under light stress, but it was not observed under… CONTINUE READING