Detection of herpes simplex virus type 1 gene expression in latently and productively infected mouse ganglia using the polymerase chain reaction.

@article{Lynas1989DetectionOH,
  title={Detection of herpes simplex virus type 1 gene expression in latently and productively infected mouse ganglia using the polymerase chain reaction.},
  author={Christopher M T.Woodworth Lynas and Keith A. Laycock and Steven W. Cook and Terry J. Hill and W. A. L. Blyth and Norman J. Maitland},
  journal={The Journal of general virology},
  year={1989},
  volume={70 ( Pt 9)},
  pages={
          2345-55
        }
}
The polymerase chain reaction (PCR) was employed to detect herpes simplex virus (HSV) sequences in the DNA, and HSV gene expression in total cell RNA, extracted from cervical and trigeminal ganglia of mice during productive and latent infection with HSV-1, strain SC16. Such gene expression was detected in 1 microgram or less of RNA, the quantity anticipated to be present in one or two cervical ganglia. Within the limits of the primers available, gene expression during latency appeared to be… CONTINUE READING

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