Detection of hepatopancreatic parvovirus in Thai shrimp Penaeus monodon by in situ hybridization, dot blot hybridization and PCR amplification.

@article{Phromjai2002DetectionOH,
  title={Detection of hepatopancreatic parvovirus in Thai shrimp Penaeus monodon by in situ hybridization, dot blot hybridization and PCR amplification.},
  author={Jurairat Phromjai and Vichai Boonsaeng and Boonsirm Withyachumnarnkul and Timothy William Flegel},
  journal={Diseases of aquatic organisms},
  year={2002},
  volume={51 3},
  pages={227-32}
}
Hepatopancreatic parvovirus (HPV) infects the hepatopancreas in penaeid shrimp and retards their growth. The DNA sequence of HPV from Thai shrimp Penaeus monodon (HPVmon) differs from HPV of Penaeus chinensis (HPVchin) by approximately 30%. In spite of this difference, commercial PCR primers (DiagXotics) developed from HPVchin to yield a 350 bp PCR product do give a 732 bp product with HPVmon DNA template. On the other hand, the sensitivity of HPVmon detection with these primers and with… CONTINUE READING

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