Detection of hepatitis C virus RNA in serum and peripheral blood mononuclear cells.

  title={Detection of hepatitis C virus RNA in serum and peripheral blood mononuclear cells.},
  author={Javier Bartolom{\'e} and Inmaculada Castillo and Juan Antonio Quiroga and Sonia Navas and Vincente Carreno},
  journal={Journal of hepatology},
  volume={17 Suppl 3},
Hepatitis C virus (HCV) RNA is the most reliable marker of HCV infection. However, because of HCV genome heterogeneity and the procedures used to isolate the viral genome and to perform the amplification steps, results do not accurately reflect the actual rate of HCV infection. In this study, several isolation procedures and sets of primers to perform PCR were compared for the detection of HCV-RNA in serum and peripheral blood mononuclear cells (PBMC) of patients with HCV infection. It was… CONTINUE READING


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