Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5'-noncoding region.

@article{Okamoto1990DetectionOH,
  title={Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5'-noncoding region.},
  author={Hiroaki Okamoto and Shigeru Okada and Yoshinori Sugiyama and Toshio Tanaka and Yoshiki Sugai and Yoshihiro Akahane and Atsuhiko Machida and Shunji Mishiro and H. Yoshizawa and Yuzo Miyakawa},
  journal={The Japanese journal of experimental medicine},
  year={1990},
  volume={60 4},
  pages={215-22}
}
The 5'-noncoding region of hepatitis C virus (HCV) genomes is highly conserved. A two-stage polymerase chain reaction (PCR), involving two pairs of primers deduced from the 5'-noncoding region of the HCV genome, was developed for a sensitive and specific detection of HCV RNA. The first stage of PCR was performed for 35 cycles with primers capable of multiplying fragments of 221 base pairs. PCR products in samples negative for HCV RNA were subjected to the second stage of PCR for 30 cycles with… CONTINUE READING

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