Detection of hepatitis B virus in serum using amplification of viral DNA by means of the polymerase chain reaction.

@article{Sumazaki1989DetectionOH,
  title={Detection of hepatitis B virus in serum using amplification of viral DNA by means of the polymerase chain reaction.},
  author={Ryo Sumazaki and Manfred Motz and Hans Wolf and Joerg Heinig and Wolfgang Jilg and Friedrich Deinhardt},
  journal={Journal of medical virology},
  year={1989},
  volume={27 4},
  pages={
          304-8
        }
}
A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedure. This assay detected viral DNA in a number of patients with proven or suspected chronic HBV infection who were all negative for HBV DNA in the conventional hybridisation assay… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 20 REFERENCES

Detection of hepatitis B sequences in serum by using in vitro enzymatic amplification

V Laure F. Courgnaud, C Rouzioux, +5 authors C Brechot
  • Journal of Virological Methods
  • 1988

An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences

R Hopkins, E Kane, +3 authors J Gitschier
  • New England Journal of Medicine
  • 1987

Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection

KB Kwok S. Mack DH. Mullis, B Poiesz, G Ehrlich, D Blair, JJ Friedman-Kien A. Sninsky
  • Journal of Virology
  • 1987

A dotblot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides

M Verlaan-deVries, ME Bogaard, JH vandenElstH.vanBoom, J vanderEbA, L BosJ
  • Gene
  • 1986

Hepatitis B virus DNA in hepatitis B surface antigen-positive blood donors: Relation to the hepatitis B e system and outcome in recipients

K Krogsgaard, J Wantzin P. Aldershvile, P Kryger, JO Anderson P. Nielsen
  • Journal of Infectious Diseases
  • 1986

Diagnostische Bedeutung des Nachweises von Hepatitis-B-Virus-DNS bei akuten und chronischen Hepatitiden

R Seelig, B Metzger, P Renz M. Metzger, HP Seelig
  • Deutsche Medizinische Wochenschrift
  • 1985