Detection of heat injury in Listeria monocytogenes Scott A.

  title={Detection of heat injury in Listeria monocytogenes Scott A.},
  author={John S. Novak and Vijay K. Juneja},
  journal={Journal of food protection},
  volume={64 11},
Methods of detecting live pathogens in foods that may be growth inhibited following heat treatment are essential to food safety. Among the techniques available, reverse transcription polymerase chain reaction (RT-PCR) amplification of messenger RNA from heat-injured Listeria monocytogenes Scott A is preferable to direct PCR in an attempt to avoid false positives from dead cells. The RT-PCR has a detection limit of 3 x 10(6) CFU/g, compared to 3 CFU/g for untreated controls, but may not be… CONTINUE READING