Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR

  title={Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR},
  author={J. F. Marinho de Araujo and A. Pereira and Marcello Schiavo Nardi and Dyana Alves Henriques and Daniele Lautenschalager and Lilia Mara Dutra and Tatiana Ometto and Renata F. Hurtado and Felipe Bernardino Rezende Mau{\'e}s and Adrian Nava and Felipe Alves Morais and Caroline Cotrin Aires and Sandra Elisa Favorito and Edison Luiz Durigon},
  journal={Archives of Virology},
Current knowledge of the pathogenic hantavirus indicates that wild rodents are its primary natural reservoir. Specific primers to detect the presence of viral genomes were developed using an SYBR-Green-based real-time RT-PCR protocol. One hundred sixty-four rodents native to the Atlantic Forest biome were captured in São Paulo State, Brazil, and their tissues were tested. The presence of hantavirus RNA was detected in sixteen rodents: three specimens of Akodon montensis, three of Akodon cursor… CONTINUE READING

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