Detection of genome DNA methylation change in spinach induced by 5-azaC.

@article{Gao2014DetectionOG,
  title={Detection of genome DNA methylation change in spinach induced by 5-azaC.},
  author={Wujun Gao and ShuFen Li and Zhongxia Li and Yingying Huang and Chuanliang Deng and Longdou Lu},
  journal={Molecular and cellular probes},
  year={2014},
  volume={28 4},
  pages={163-6}
}
DNA methylation has been implicated in the regulation of gene expression, genome imprinting, and chromatin remodeling in eukaryotes. In this study, we analyzed possible alterations in levels and patterns of cytosine methylation in male and female spinach plants after treatment with demethylation agent 5-azacytidine (5-azaC) using two methods: (1) direct determination of 5-methylcytidine (5 mC) amounts in genomic DNA by high-performance liquid chromatography (HPLC) separation and quantification… CONTINUE READING