Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR.

@article{Herrewegh1995DetectionOF,
  title={Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR.},
  author={Arnold Herrewegh and Raoul J de Groot and Arnost Cepica and Herman F. Egberink and Marian C. Horzinek and Peter J. M. Rottier},
  journal={Journal of clinical microbiology},
  year={1995},
  volume={33 3},
  pages={
          684-9
        }
}
A nested reverse transcriptase PCR (RT-nPCR) was developed for the detection of feline coronavirus (FCoV) RNA in the feces, tissues, and body fluids of infected cats. The RT-nPCR was targeted to the highly conserved 3'-untranslated region of the viral genome and will detect most, if not all, feline coronaviruses in the field. With the RT-nPCR, FCoV RNA was detected in plasma samples from experimentally infected cats as early as 2 days postinoculation. FCoV RNA was also detected in serum, plasma… CONTINUE READING
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