Detection of fecal bacteria and source tracking identifiers in environmental waters using rRNA-based RT-qPCR and rDNA-based qPCR assays.

@article{Pitknen2013DetectionOF,
  title={Detection of fecal bacteria and source tracking identifiers in environmental waters using rRNA-based RT-qPCR and rDNA-based qPCR assays.},
  author={Tarja Pitk{\"a}nen and H. T. Ryu and Michael R. Elk and Anna-Maria Hokaj{\"a}rvi and Sallamaari Siponen and Asko Veps{\"a}l{\"a}inen and Pia R{\"a}s{\"a}nen and Jorge W. Santo Domingo},
  journal={Environmental science & technology},
  year={2013},
  volume={47 23},
  pages={13611-20}
}
In this study, we evaluated the use of RT-qPCR assays targeting rRNA gene sequences for the detection of fecal bacteria in water samples. We challenged the RT-qPCR assays against RNA extracted from sewage effluent (n = 14), surface water (n = 30), and treated source water (n = 15) samples. Additionally, we applied the same assays using DNA as the qPCR template. The targeted fecal bacteria were present in most of the samples tested, although in several cases, the detection frequency increased… CONTINUE READING