Detection of estrogen receptor mRNA in human uterus.

  title={Detection of estrogen receptor mRNA in human uterus.},
  author={Fritz F. Parl and Christopher P Schonbaum and David L. Cox and Douglas R Cavener},
  journal={Molecular and cellular endocrinology},
  volume={52 3},
We transcribed a cDNA clone of the human estrogen receptor (ER) with T7 RNA polymerase. The 32P-cRNA transcript complementary to ER mRNA was hybridized to poly(A)+ RNA from human uterus and revealed a single band of approximately 4.2 kilobases. No hybridization was seen with the cRNA probe of the opposite orientation. Hybridization of total RNA from calf and rat uterus yielded a single band at approximately 3.8 kilobases for both species. Total RNA from rat spleen did not hybridize. A 35S… CONTINUE READING

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