Detection of essential arginine in bacterial peptidyl dipeptidase-4: arginine is not the anion binding site.

@article{Lanzillo1989DetectionOE,
  title={Detection of essential arginine in bacterial peptidyl dipeptidase-4: arginine is not the anion binding site.},
  author={Joseph J. Lanzillo and Yamuna Dasarathy and Barry L. Fanburg},
  journal={Biochemical and biophysical research communications},
  year={1989},
  volume={160 1},
  pages={243-9}
}
Peptidyl dipeptidase-4 from Pseudomonas maltophilia was modified with the arginine reagents p-hydroxyphenylglyoxal and 2,3-butanedione. The enzyme was inactivated in a pseudo-first-order manner by p-hydroxyphenylglyoxal with a half-time of 72 min. Inactivation by 2,3-butanedione was biphasic with a rapid phase followed by a slower inactivation to less than… CONTINUE READING