Detection of epizootic hemorrhagic disease virus in Culicoides variipennis (Diptera: Ceratopogonidae).

@article{Wilson1991DetectionOE,
  title={Detection of epizootic hemorrhagic disease virus in Culicoides variipennis (Diptera: Ceratopogonidae).},
  author={William C. Wilson},
  journal={Journal of medical entomology},
  year={1991},
  volume={28 5},
  pages={742-4}
}
Nucleic acid hybridization was used to detect epizootic hemorrhagic disease (EHD) virus serotype 1 and serotype 2 in Culicoides variipennis (Coquillett). Adult females were inoculated intrathoracically with virus, then were assayed daily for the presence of viral RNA for 2 wk, at which time maximum virus replication is likely to occur. Viral RNA of EHD serotypes 1 and 2 was first detected by hybridization on days 9 and 7 after infection, respectively, and then for up to 14 days after infection… CONTINUE READING