Detection of enzymatic activity of transfer RNA modification enzymes using radiolabeled tRNA substrates.

@article{Grosjean2007DetectionOE,
  title={Detection of enzymatic activity of transfer RNA modification enzymes using radiolabeled tRNA substrates.},
  author={Henri Grosjean and Louis Droogmans and Martine Roovers and G{\'e}rard Keith},
  journal={Methods in enzymology},
  year={2007},
  volume={425},
  pages={55-101}
}
The presence of modified ribonucleotides derived from adenosine, guanosine, cytidine, and uridine is a hallmark of almost all cellular RNA, and especially tRNA. The objective of this chapter is to describe a few simple methods that can be used to identify the presence or absence of a modified nucleotide in tRNA and to reveal the enzymatic activity of particular tRNA-modifying enzymes in vitro and in vivo. The procedures are based on analysis of prelabeled or postlabeled nucleotides (mainly with… CONTINUE READING
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