Detection of enterovirus capsid protein VP1 in myocardium from cases of myocarditis or dilated cardiomyopathy by immunohistochemistry: further evidence of enterovirus persistence in myocytes

  title={Detection of enterovirus capsid protein VP1 in myocardium from cases of myocarditis or dilated cardiomyopathy by immunohistochemistry: further evidence of enterovirus persistence in myocytes},
  author={Hongyi Zhang and Yanwen Li and Dougal Mcclean and Peter J. Richardson and R. A. Florio and Mary N. Sheppard and Karen Morrison and Najma Latif and Michael J. Dunn and L. C. Archard},
  journal={Medical Microbiology and Immunology},
The association of enteroviruses with myocardial disease has been investigated extensively by molecular biological techniques to detect viral RNA, but remains controversial. This retrospective study investigated the involvement of enterovirus in myocarditis or dilated cardiomyopathy (DCM) by detection of viral antigens in myocardial samples from a new patient series using an optimized immunohistochemical technique. Formalin-fixed, paraffin-embedded biopsy, autopsy or explanted myocardial tissue… 
Screening of Coxsackie B virus infection among patients with myocarditis and dilated cardiomyopathy using Serion Elisa classic Coxsackie virus IgM in Chennai, Tamilnadu, South India
Almost 70% of the myocarditis and dilated cardiomyopathy (DCM) cases are found to be caused by Enterovirus genus, the majority of these infections were caused by coxsackie virus B (CVB) group. There
Persistent coxsackievirus infection: enterovirus persistence in chronic myocarditis and dilated cardiomyopathy.
  • N. Chapman, K. Kim
  • Biology, Medicine
    Current topics in microbiology and immunology
  • 2008
Factors that control the extent of cardiac infection with terminally deleted enteroviruses and the relative roles of continued immune response of the virus vs viral modification of cardiac function need to be measured to find effective therapies for the human disease.
Type 1 Diabetes Is Associated With Enterovirus Infection in Gut Mucosa
It is suggested that a large proportion of type 1 diabetic patients have prolonged/persistent enterovirus infection associated with an inflammation process in gut mucosa, which opens new opportunities for studying the viral etiology ofType 1 diabetes.
Detection of enterovirus protein and RNA in multiple tissues from nPOD organ donors with type 1 diabetes
It is hypothesize that extra-pancreatic organs and their interplay with EV in T1D pathogenesis remains to be solved, but it is hypothesized that these organs may serve as a reservoir for the virus which may reside in these tissues in a slow-replicating persistent form.
Immunohistochemical diagnosis of human infectious diseases: a review
The methodological evolutions of IHC and its application to the diagnosis of infectious diseases, more specifically lice-borne diseases, sexually transmitted diseases and skin infections, were critically examined and it was found that IHC plays an important role in diagnostic of infectious Diseases in tissue samples.
Global study of viral myocarditis: A systematic review and meta-analysis
Treatment of myocarditis is still problematic and may depend on the etiologic diagnosis, so it is important to know the commonly occurring viral factors inMyocarditis and timely diagnosis and treatment are also imperative.
Detection of Non-Polio Enteroviruses From 17 Years of Virological Surveillance of Acute Flaccid Paralysis in the Philippines
Despite some limitations in this study, plural NPEVs with multiple patterns of circulation in the Philippines for 17 years were identified.
Enteroviruses and
Infections with enteroviruses and human parechovirus infections are highly prevalent, particularly in neonates, where they may cause substantial morbidity and mortality, and are seasonal in nature.
Synergy of molecular and serological methods in minimally invasive diagnosis of enteroviral cardiac infection.
The synergistic use of minimally invasive serological, IgG, IgM, IgA, and neutralizing titres, and RNA detection was evaluated on representative patients out of 238 suffering from cardiopathies to highlight the presence of a viral aetiology so that an endomyocardial biopsy can be performed thus eliminating incorrect therapies.


Enteroviral capsid protein VP1 is present in myocardial tissues from some patients with myocarditis or dilated cardiomyopathy.
The findings demonstrate a direct link between enterov virus infection and some myocarditis or DCM cases and suggest that viral protein synthesis may be involved in persistent enterovirus infection in the pathogenesis of DCM.
Localization of Enteroviral Antigen in Myocardium and Other Tissues from Patients with Heart Muscle Disease by an Improved Immunohistochemical Technique
  • H. Zhang, Y. Li, L. Archard
  • Biology, Medicine
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • 2000
An immunohistochemical technique was optimized to investigate the expression of viral capsid proteins in situ and can be used for a definitive laboratory diagnosis of enterovirus-associated diseases and for studying the mechanisms of virus persistence in chronic myocardial disease.
Clinical and prognostic significance of detection of enteroviral RNA in the myocardium of patients with myocarditis or dilated cardiomyopathy.
It is demonstrated that the detection ofEnterovirus RNA in the myocardium of patients with heart muscle disease at the time of initial investigation is associated with an adverse prognosis and that the presence of enteroviral RNA is an independent predictor of clinical outcome.
Enterovirus RNA Replication in Cases of Dilated Cardiomyopathy: Light Microscopic In Situ Hybridization and Virological Analyses of Myocardial Specimens Obtained at Partial Left Ventriculectomy
It is suggested that active enteroviral replication plays a role in the development of myocardial lesions in DCM patients, and appears to be a prognostic factor for DCM after PLV.
Enteroviruses can persist with or without active viral replication in cardiac tissue of patients with end-stage ischemic or dilated cardiomyopathy.
It is demonstrated that a small percentage of patients with end-stage chronic cardiac diseases had active enterovirus replication in their myocardium.
High prevalence of enteroviral genomic sequences in myocardium from cases of endemic cardiomyopathy (Keshan disease) in China
Nucleotide sequence data suggest that coxsackievirus B or coxsackingie B like viruses are often involved in Keshan disease, and results support an aetiological role for enteroviral infection in Kshan disease.
Absence of enteroviral RNA in hearts explanted from patients with dilated cardiomyopathy.
A search for the presence of the enteroviral capsid protein VP1 in pancreases of patients with Type 1 (insulin-dependent) diabetes and pancreases and hearts of infants who died of coxsackieviral myocarditis
The continuing destruction of insulin-secreting B cells seen at the time of death in the diabetic pancreas is unlikely to be due to a direct cytopathic effect of a coxsackie B virus, but the possibility that a persistent infection of B cells by a defective enterovirus may result in their destruction by an autoimmune mechanism is not excluded.
Failure to demonstrate enterovirus aetiology in Swedish patients with dilated cardiomyopathy
Attempts were made to establish a possible relationship between enterovirus infection and dilated cardiomyopathy (DCM) by use of serology, virus isolation from faecal samples, and detection of