Detection of enterotoxigenic Bacteroides fragilis by PCR.

@article{Pantosti1997DetectionOE,
  title={Detection of enterotoxigenic Bacteroides fragilis by PCR.},
  author={A Pantosti and Monica Malpeli and M Wilks and Maria Grazia Menozzi and Fabrizio D'Ambrosio},
  journal={Journal of clinical microbiology},
  year={1997},
  volume={35 10},
  pages={2482-6}
}
Strains of enterotoxigenic Bacteroides fragilis (ETBF) are associated with diarrhea in young farm animals and, at least in particular settings, in children. Enterotoxin production by ETBF is currently detected by a tissue culture assay with HT-29 cells. We have developed a PCR assay based on the detection of the enterotoxin gene to identify ETBF in culture and in stool samples. Overall, 113 bacterial strains were examined, including 3 B. fragilis reference strains, 75 B. fragilis isolates… CONTINUE READING

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