Detection of endogenous salsolinol in neonatal rat tissue by a radioenzymatic--thin-layer chromatographic assay.

Abstract

A sensitive radioenzymaticc--thin-layer chromatographic assay for the quantitative analysis of the tetrahydroisoquinoline alkaloid, salsolinol, in plasma and neonatal rat tissue is described. The assay involves the enzymatic O-methylation of salsolinol by catechol-O-methyltransferase in presence of [3H]S-adenosylmethionine, and subsequent separation by thin… (More)

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