Detection of early pregnancy-specific proteins in Holstein milk.

  title={Detection of early pregnancy-specific proteins in Holstein milk.},
  author={R. Han and Hong-Rye Kim and Y. Diao and Min Gu Lee and D. Jin},
  journal={Journal of proteomics},
  volume={75 11},
Bovine pregnancy is commonly diagnosed by rectal palpation or ultrasonography and changes in progesterone concentration. To determine a simpler and less expensive diagnostic method, we sought to identify early pregnancy-specific proteins in bovine milk by comparing samples collected from pregnant and non-pregnant Holstein cattle. Of the 600-700 protein spots visible on 2-DE gel images, 39 were differentially expressed in milk from pregnant and non-pregnant cattle. Antibodies generated against… Expand
Pregnancy-associated glycoprotein profile in milk and its relationship with the circulating level during early pregnancy in goats
The strong relationship and high agreement of milk PAG with its level in peripheral circulation supports that milk is a good alternative to plasma for early pregnancy diagnosis from d 28 to d 51 and can effectively be used as a marker of placental functions and fetal health in goats. Expand
Differences in the bovine milk whey proteome between early pregnancy and the estrous cycle.
The objective of the present study was to use liquid chromatography tandem mass spectrometry (LC-MS/MS) to perform a global, label-free, proteomics study on milk whey and extracellular vesicle (EV) enriched milk Whey samples, in order to identify potential protein biomarkers of early pregnancy. Expand
Bovine milk sampling efficiency for pregnancy-associated glycoproteins (PAG) detection test
It was found that the time of milking did not affect the PAG levels and the occurrence of carryover in the milk meter, and a single milk sample can be used for both milk quality tests and pregnancy test. Expand
Recent Possibilities for the Diagnosis of Early Pregnancy and Embryonic Mortality in Dairy Cows
  • O. Szenci
  • Medicine
  • Animals : an open access journal from MDPI
  • 2021
Although the early pregnancy factor is the earliest specific indicator of fertilization, at present, its detection is entirely dependent on the use of the rosette inhibition test; therefore, its use in the field needs further developments. Expand
Storage strategies and processing of bovine milk samples for pregnancy-associated glycoproteins detection test
ABSTRACT Three experiments were performed to test if the time and storage conditions of milk samples, the preheating of samples in a water bath, as well as the carryover effect in laboratory analysisExpand
Expanding the bovine milk proteome through extensive fractionation.
An extensive preanalysis fractionation of raw composite milk collected from documented healthy cows in early lactation expanded the milk proteome by applying different fractionation techniques to milk, and may serve as a reference for scientists working in the dairy sector. Expand
Application of proteomics to the areas of milk production, processing and quality control – A review
The use of proteomics is described in studies on milk proteins in relation to species–species differences, post-translational modification, effect of lactation, mastitis, changes in heated milk and during cheese ripening, and the detection of milk adulteration. Expand


Pregnancy-associated glycoproteins (PAG) detection in milk samples for pregnancy diagnosis in dairy goats.
A pregnancy diagnosis based on milk concentrations of PAG, that could be of great use on the farm, is reported for the first time. Expand
Accuracy of pregnancy specific protein-B test for early pregnancy diagnosis in dairy cattle.
The objective was to evaluate the accuracy of an ELISA for pregnancy specific protein B (PSP-B) for early pregnancy diagnosis in dairy cattle. Blood from lactating (>100 d postpartum) dairy cows (n =Expand
Concentration of bovine pregnancy associated glycoprotein in plasma and milk: its application for pregnancy diagnosis in cows.
The data showed that the RIA method is precise enough to measure PAG concentrations in the maternal blood and milk of cows and indicates that milk samples can be used for pregnancy diagnosis in cows. Expand
Pregnancy - associated glycoproteins as a new diagnostic tool in cattle reproduction.
Specific RIA tests were developed (classic and alternative RIA) and used to measure the concentration of these glycoproteins in blood during gestation and the postpartum period in cattle. Expand
Diagnosis of pregnancy by radioimmunoassay of a pregnancy-specific protein in the plasma of dairy cows.
It is concluded that 30 days after AI, the bPSPB RIA is an efficient test both for pregnancy prediction and detection of nonpregnant dairy cows. Expand
Comparison of commercial ELISA blood tests for early pregnancy detection in dairy cows.
Two blood pregnancy assays were equally efficacious and were highly accurate (based on transrectal ultrasonography as the gold standard) for early pregnancy diagnosis in dairy cattle using trans rectal ultr Masonography as a gold standard. Expand
Comparison of pregnancy diagnosis in dairy cattle by use of a commercial ELISA and palpation per rectum.
Good agreement existed between the 2 pregnancy tests in dairy cattle, especially at longer intervals after insemination, and Discrepant results appeared to be attributable to a nonviable fetus, embryonic loss, or fetal loss. Expand
Detection of pregnancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation.
In a commercial herd of 102 beef cows, the assay could detect pregnancy earlier and more accurately than the routine method of rectal palpation and provides a serological method for detecting pregnancy in cows. Expand
Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses.
The findings suggest that PAG concentrations during the early fetal period are related to the day of gestation, milk production, number of fetuses and sire of fetus in high producing dairy cows. Expand
Early pregnancy diagnosis in cattle by means of linear-array real-time ultrasound scanning of the uterus and a qualitative and quantitative milk progesterone test.
Three methods for diagnosing early pregnancy in cattle are compared: 1) a trans-rectal ultrasound scan of the uterus, 2) a cow-side enzymeimmunoassay (EIA) milk progesterone test, and 3) a radioimmunosorbent test, which focuses on the effects of early embryonic death and the accumulation of intrauterine fluids. Expand