Detection of differential expression of porcine IFN-alpha subtypes by reverse transcription polymerase chain reaction.

@article{Cheng2007DetectionOD,
  title={Detection of differential expression of porcine IFN-alpha subtypes by reverse transcription polymerase chain reaction.},
  author={Gong Cheng and Xin Zhao and Weizao Chen and Weiyao Yan and Mingqiu Liu and Jie Chen and Zhaoxin Zheng},
  journal={Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research},
  year={2007},
  volume={27 7},
  pages={579-87}
}
The porcine interferon-alpha (IFN-alpha) multigene family is a new IFN-alpha system in recent research. Characterization of the PoIFN-alpha multigene family has been described in our previous work, and 14 functional PoIFN-alpha genes were detected in the porcine genome. In this study, we designed subtype-specific primers and consensus primers for PoIFN-alpha. The expression of PoIFN-alpha was detected using the two PCR strategies in three systems, namely, poly(I).poly(C)-DEAE-dextran-induced… CONTINUE READING

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