Detection of dengue virus genome in urine by real-time reverse transcriptase PCR: a laboratory diagnostic method useful after disappearance of the genome in serum.

@article{Hirayama2012DetectionOD,
  title={Detection of dengue virus genome in urine by real-time reverse transcriptase PCR: a laboratory diagnostic method useful after disappearance of the genome in serum.},
  author={Takanori Hirayama and Yasutaka Mizuno and Nozomi Takeshita and Akira Kotaki and Shigeru Tajima and Tsutomu Omatsu and Kouichi Sano and Ichiro Kurane and Tomohiko Takasaki},
  journal={Journal of clinical microbiology},
  year={2012},
  volume={50 6},
  pages={2047-52}
}
The reemergence of dengue virus (DENV) infection has created a requirement for improved laboratory diagnostic procedures. In this study, DENV genome detection in urine was evaluated as a diagnostic method. The DENV genome was detected by real-time reverse transcriptase PCR (RT-PCR) in urine and serum of dengue patients. The detection rate of DENV genome in urine was 25% (2/8) on disease days 0 to 3 and 32% (7/22) on days 4 to 5. The rate was 50% or higher on days 6 to 16, 52% (11/21) on days 6… CONTINUE READING
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