Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis.

@article{Ulfgren1995DetectionOC,
  title={Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis.},
  author={A K Ulfgren and Staffan Lindblad and Lars Klareskog and Jan Andersson and Ulf Andersson},
  journal={Annals of the rheumatic diseases},
  year={1995},
  volume={54 8},
  pages={654-61}
}
OBJECTIVES To develop and evaluate a new immunohistochemical method to study the localisation and phenotype of individual cytokine producing cells in synovial biopsy specimens in rheumatoid arthritis. METHODS Cryopreserved sections of synovial tissue from nine patients with rheumatoid arthritis were incubated with carefully selected cytokine specific antibodies detecting 19 different cytokines, after fixation of the specimens with paraformaldehyde and using saponin to permeabilise the cell… CONTINUE READING

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