Detection of cross-links between FtsH, YidC, HflK/C suggests a linked role for these proteins in quality control upon insertion of bacterial inner membrane proteins.

@article{Bloois2008DetectionOC,
  title={Detection of cross-links between FtsH, YidC, HflK/C suggests a linked role for these proteins in quality control upon insertion of bacterial inner membrane proteins.},
  author={Edwin van Bloois and Henk L. Dekker and Linda Fr{\"o}derberg and Edith N G Houben and Malene L. Urbanus and Chris G. de Koster and Jan-Willem de Gier and Joen Luirink},
  journal={FEBS letters},
  year={2008},
  volume={582 10},
  pages={1419-24}
}
Little is known about the quality control of proteins upon integration in the inner membrane of Escherichia coli. Here, we demonstrate that YidC and FtsH are adjacent to a nascent, truncated membrane protein using in vitro photo cross-linking. YidC plays a critical but poorly understood role in the biogenesis of E. coli inner membrane proteins (IMPs). FtsH functions as a membrane chaperone and protease. Furthermore, we show that FtsH and its modulator proteins HflK and HflC copurify with tagged… CONTINUE READING