Detection of coproantigens by sandwich ELISA in sheep experimentally infected with Fasciola gigantica.

@article{Estuningsih2004DetectionOC,
  title={Detection of coproantigens by sandwich ELISA in sheep experimentally infected with Fasciola gigantica.},
  author={S Endah Estuningsih and S. Widjayanti and G Adiwinata and David Piedrafita},
  journal={Tropical biomedicine},
  year={2004},
  volume={21 2},
  pages={
          51-6
        }
}
Two monoclonal antibodies to Fasciola gigantica excretory/secretory (ES) antigens were used in a sandwich ELISA for the detection of Fasciola antigens in faeces of 9 sheep experimentally infected with 300 metacercariae of F. gigantica. The detection of coproantigens was found in four of the seven sheep within 5 weeks of infection, and within 7 weeks of infection coproantigens were detected in all seven of the sheep. This technique was compared to an indirect ELISA for the detection of anti… CONTINUE READING

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